https://ogma.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:34323 Rhodopseudomonas palustris C1 > R. palustris AB3 > Rubrivivax benzoatilyticus C31 > R. palustris L28 which corresponded to the presence of As‐resistance genes in these bacteria. The strain C1 showed all As‐marker genes; arsC, arsM, aioA, and acr3, while aioA was not detected in strain AB3. Strains C31 and L28 had only Arsenite‐transporter gene, acr3. Translation of all these detected gene sequences of strain C1 to amino acid sequences showed that these proteins have vicinal cysteine; Cys126, Cys105, and Cys178 of Acr3, ArsC, AioA, respectively. Tertiary structure of proteins Acr3, ArsC, AioA, and ArsM showed strain C1 exhibits the high activities of arsenite oxidase and arsenate reductase enzymes that are encoded by aioA and arsC genes, respectively. Moreover, strain C1 with arsM gene produced volatile‐methylated As‐compounds; monomethylarsonic acid (MMA), dimethylarsenic acid (DMA), and arsenobetaine (AsB) in the presence of either As(III) or As(V). In conclusion, the strain C1 has great potential for its application in bioremediation of As‐contaminated sites.]]> Wed 27 Feb 2019 12:32:39 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:24068 Azospirillum sp. based on phylogenetic analysis of 16S rRNA. The strain oxidized 100 µM As(III) to As(V) in both culture media (minimal salts) and real groundwater within 8 and 10 h, respectively. The oxidation of As(III) by this strain was observed within the pH range 5-10 with the best performance at pH 7-8. As(III) oxidation was found to be independent of cell growth which implies the oxidation enzymes are constitutively expressed. The whole cell kinetic study highlighted a lower value of kinetic constant, K_s as 32.9 µM As(III), which indicates that the strain MM-17 has greater affinity for As(III). The gene sequence of the large subunit of arsenite oxidase of MM-17 showed 99 and 72% similarity to the large subunit of arsenite oxidase of Stenotrophomonas sp. MM-7 and Sinorhizobium sp. M14, respectively. Sphaeroplasts experiments suggest that arsenite oxidase is a membrane associated protein in MM-17.]]> Sat 24 Mar 2018 07:09:39 AEDT ]]>